5W6W
Designing Higher Resolution Self-Assembled 3D DNA Crystals via Strand Terminus Modifications
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 106.712, 106.712, 94.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.960 - 3.060 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.451 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.107 | 0.431 |
| Number of reflections | 5152 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 68.0 | 19.2 |
| Redundancy | 7.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Grown by vapor diffusion while treated with a controlled temperature gradient from 333K degs to 293K. They were grown in Ammonium Sulfate, TRIS, Acetic Acid and EDTA. |
