5W6L
Crystal Structure of RRSP, a MARTX Toxin Effector Domain from Vibrio vulnificus CMCP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 247.103, 247.103, 247.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 3.450 |
| R-factor | 0.22888 |
| Rwork | 0.228 |
| R-free | 0.24913 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.510 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.113 | 0.866 |
| Rmeas | 0.046 | 0.382 |
| Number of reflections | 33078 | 1638 |
| <I/σ(I)> | 18.4 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.8 | 5.9 |
| CC(1/2) | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Protein: 15.6 mg/ml, 0.5M Sodium chloride, 0.01M Tris HCl (pH 8.3); Screen: 1.96M Ammonium sulfate, 0.1 MOPS (pH 6.5), 20% (v/v) PEG400; Cryo: 4M Formate |
