5W53

Crystal structure of the erythrocyte-binding domain from Plasmodium vivax reticulocyte-binding protein 2b (PvRBP2b)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	59.500, 124.200, 65.310
Unit cell angles	90.00, 97.75, 90.00

Refinement procedure

Resolution	34.170 - 1.710
R-factor	0.168
Rwork	0.168
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z8n
RMSD bond length	0.014
RMSD bond angle	1.321
Data reduction software	iMOSFLM (1.0.7)
Data scaling software	Aimless (0.2.7)
Phasing software	PHASER
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.870	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.111	1.711
Number of reflections	100494
<I/σ(I)>	10	1.2
Completeness [%]	99.4	98.8
Redundancy	7.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	22% W/V PEG3350, 200 MM POTASSIUM THIOCYANATE, 100 MM BIS-TRIS-HCL, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K