5W42

Crystal structure of human monoclonal antibody H3v-47 in complex with influenza virus hemagglutinin from A/Minnesota/11/2010 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 21 3
Unit cell lengths	194.622, 194.622, 194.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.924 - 3.569
R-factor	0.198
Rwork	0.196
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp2
RMSD bond length	0.013
RMSD bond angle	1.988
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.630
High resolution limit [Å]	3.569	3.570
Rmerge	0.112	0.784
Rpim	0.045	0.584
Number of reflections	29479	1441
<I/σ(I)>	14.8
Completeness [%]	100.0	100
Redundancy	13.1	10.1
CC(1/2)		0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	10% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.1M MES (pH 5.0)