5W2J

Crystal structure of dimeric form of mouse Glutaminase C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	293
Detector technology	CCD
Collection date	2011-10-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	84.940, 100.880, 145.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
Rwork	0.196
R-free	0.22761
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.890
High resolution limit [Å]	2.300	2.300
Number of reflections	51086
<I/σ(I)>	50.9
Completeness [%]	92.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	293.15	9% PEG8000, 10% glycerol, 50 mM Tris-HCl, pH 8.5