5W0W
Crystal structure of Protein Phosphatase 2A bound to TIPRL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 32 |
| Unit cell lengths | 150.983, 150.983, 285.498 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.421 - 3.800 |
| R-factor | 0.2002 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.678 |
| Data reduction software | XDS |
| Data scaling software | Aimless (1.10.13) |
| Phasing software | CRANK (2.0.11) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.880 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.875 | 3.290 |
| Rpim | 0.273 | 1.000 |
| Number of reflections | 143440 | 4467 |
| <I/σ(I)> | 11.3 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 292 | 0.1M citric acid pH 5.2, 9% PEG4000, 0.2M sodium acetate |
