5W0P

Crystal structure of rhodopsin bound to visual arrestin determined by X-ray free electron laser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	273
Detector technology	PIXEL
Collection date	2014-11-06
Detector	CS-PAD CXI-1
Wavelength(s)	1.33
Spacegroup name	P 21 21 21
Unit cell lengths	109.240, 109.240, 452.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.030 - 3.013
R-factor	0.2369
Rwork	0.234
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zwj
RMSD bond length	0.005
RMSD bond angle	0.845
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	76360
<I/σ(I)>	4.5
Completeness [%]	72.4
Redundancy	287
CC(1/2)	0.988	0.233

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	PEG 400, magnesium acetate, sodium acetate.