5W0N

Structure of human TUT7 catalytic module (CM) in complex with UMPNPP and U2 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61
Unit cell lengths	135.972, 135.972, 181.574
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.878 - 2.497
R-factor	0.1881
Rwork	0.187
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0b
RMSD bond length	0.005
RMSD bond angle	0.585
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.798	2.510
High resolution limit [Å]	2.497	2.497
Rmerge	0.063
Number of reflections	65579
<I/σ(I)>	15.5
Completeness [%]	99.6
Redundancy	4.4
CC(1/2)	0.998	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.8-1.0 M lithium sulfate, 0.075-0.2 M potassium iodide, 0.05-0.2 M trisodium citrate, pH 5-6