5VYT

Crystal structure of the WbkC N-formyltransferase (F142A variant) from Brucella melitensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-23
Detector	Bruker Platinum 135
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	67.315, 68.021, 236.035
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.20537
Rwork	0.202
R-free	0.26779
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vyr
RMSD bond length	0.013
RMSD bond angle	1.664
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.093	0.412
Number of reflections	54309	5278
<I/σ(I)>	7.1	1.8
Completeness [%]	96.5	80.6
Redundancy	6.3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	13-15% PEG5000, 2% glycerol, 5 mM GDP-perosamine, 5 mM folinic acid, 100 mM MES