5VYD
Crystal structure of phosphodiesterase domain of RhoPDE fusion protein from the Choanoflagellate Salpingoeca rosetta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.649, 96.400, 108.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.986 - 2.300 |
R-factor | 0.2177 |
Rwork | 0.216 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1taz |
RMSD bond length | 0.002 |
RMSD bond angle | 0.523 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | PHENIX (dev_2719) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.129 | 0.794 |
Number of reflections | 34788 | 4804 |
<I/σ(I)> | 12.9 | 2.8 |
Completeness [%] | 98.0 | 94.8 |
Redundancy | 12.2 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 15% PEG3350, 100 mM succinic acid |