5VXA

Structure of the human Mesh1-NADPH complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	47.780, 80.370, 114.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.070 - 2.100
R-factor	0.1675
Rwork	0.165
R-free	0.20550
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.070
High resolution limit [Å]	2.100
Number of reflections	26452
<I/σ(I)>	17.1
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	4.5 mg/mL hMesh1 D66K, 50 mM NADPH, 25 mM Tris (pH 8.0), 100 mM NaCl, 0.05% 2-mercaptoethanol, 0.5 mM ZnCl2, 100 mM ammonium acetate, 50 mM sodium citrate, 12.5% PEG 4000.