5VWV

Bak core latch dimer in complex with Bim-BH3 - Cubic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 41 3 2
Unit cell lengths	139.404, 139.404, 139.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.032 - 1.897
R-factor	0.1579
Rwork	0.157
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.755
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.032	1.966
High resolution limit [Å]	1.897	1.898
Rmerge	0.216
Number of reflections	37157	3647
<I/σ(I)>	24.26	1.48
Completeness [%]	100.0	100
Redundancy	47.8	47
CC(1/2)		0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	18 % glycerol, 21.6 % PEG (poly-ethylene glycol) 1500 and 0.5 % ethyl acetate