5VU8

TNA polymerase, closed ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	72.288, 107.360, 147.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.424 - 3.200
R-factor	0.2608
Rwork	0.258
R-free	0.29460
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.424	3.290
High resolution limit [Å]	3.200	3.200
Rmerge	0.275	1.100
Number of reflections	19544
<I/σ(I)>	9.7
Completeness [%]	99.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.2 M sodium sulfate, 0.1 M MES, pH 4.0, 3% w/v 1,6-hexanediol, 19% PEG3350