5VTD
Crystal Structure of the Co-bound Human Heavy-Chain Ferritin variant 122H-delta C-star
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-06 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 180.244, 180.244, 180.244 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.720 - 1.950 |
R-factor | 0.178 |
Rwork | 0.174 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cmq |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.720 | 2.030 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.132 | 0.607 |
Number of reflections | 18852 | |
<I/σ(I)> | 10.9 | 2 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 8.1 | 4.2 |
CC(1/2) | 0.991 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | Reservoir: 500 uL total volume: 25 mM Tris (pH 8), 12 mM CaCl2, 150 mM NaCl, 0.3 mM CoCl2, 1% PEG 1900 MME Sitting Drop: 2 uL reservoir, 2 uL of 4 uM ferritin |