5VT2
Crystal structure of growth differentiation factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.720, 64.720, 172.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.2083 |
| Rwork | 0.206 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r52 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.643 |
| Data reduction software | MOSFLM (7.0.5) |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.139 | 10.000 | 2.410 |
| High resolution limit [Å] | 2.282 | 7.220 | 2.300 |
| Rmerge | 0.041 | 0.704 | |
| Rmeas | 0.090 | 0.047 | 0.797 |
| Rpim | 0.040 | 0.023 | 0.366 |
| Number of reflections | 19823 | ||
| <I/σ(I)> | 11.3 | 13.8 | 1.1 |
| Completeness [%] | 99.8 | 98.5 | 99 |
| Redundancy | 5 | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 15% w/v Tascimate, pH 7.0, 0.1 M HEPES, pH 7.0, 2% PEG3350 |
