5VSU

Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	70.157, 114.728, 179.844
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.723 - 3.100
R-factor	0.2386
Rwork	0.234
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.667
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.723	3.290
High resolution limit [Å]	3.100	3.100
Rmerge	0.250	4.031
Number of reflections	27164
<I/σ(I)>	13	1.2
Completeness [%]	100.0	100
Redundancy	51.2	47.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350