5VSN

Crystal structure of mouse ryanodine receptor 2 SPRY2 domain (1080-1253) disease mutant P1124L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 2 21 21
Unit cell lengths	36.290, 66.230, 66.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.611 - 1.439
R-factor	0.1746
Rwork	0.174
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p9i
RMSD bond length	0.005
RMSD bond angle	0.757
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.460
High resolution limit [Å]	1.439	3.910	1.440
Rmerge	0.109	0.077	0.830
Rmeas	0.118	0.084	0.918
Rpim	0.045	0.033	0.384
Number of reflections	29741
<I/σ(I)>	11.7
Completeness [%]	99.9	98.6	99.9
Redundancy	6.7	6.3	5.2
CC(1/2)		0.992	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M potassium thiocyanate, 30% PEG2000 MME