5VSN
Crystal structure of mouse ryanodine receptor 2 SPRY2 domain (1080-1253) disease mutant P1124L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 36.290, 66.230, 66.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.611 - 1.439 |
R-factor | 0.1746 |
Rwork | 0.174 |
R-free | 0.19260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p9i |
RMSD bond length | 0.005 |
RMSD bond angle | 0.757 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.460 |
High resolution limit [Å] | 1.439 | 3.910 | 1.440 |
Rmerge | 0.109 | 0.077 | 0.830 |
Rmeas | 0.118 | 0.084 | 0.918 |
Rpim | 0.045 | 0.033 | 0.384 |
Number of reflections | 29741 | ||
<I/σ(I)> | 11.7 | ||
Completeness [%] | 99.9 | 98.6 | 99.9 |
Redundancy | 6.7 | 6.3 | 5.2 |
CC(1/2) | 0.992 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M potassium thiocyanate, 30% PEG2000 MME |