5VSK

Structure of DUB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	72.390, 69.630, 77.970
Unit cell angles	90.00, 99.16, 90.00

Refinement procedure

Resolution	44.154 - 3.330
R-factor	0.2211
Rwork	0.218
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.482
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.650	48.660	3.390
High resolution limit [Å]	3.330	9.020	3.330
Rmeas	0.070	0.033	0.997
Rpim	0.038	0.018	0.550
Total number of observations	37783
Number of reflections	11226
<I/σ(I)>	13.6	35.3	1.6
Completeness [%]	98.5	98.7	98
Redundancy	3.4	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, NaFormate