5VR8
Human UDP-Glucose Dehydrogenase with UDP-Xylose Bound to the Co-enzyme Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.080, 196.490, 111.260 |
| Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
| Resolution | 49.123 - 1.999 |
| R-factor | 0.1578 |
| Rwork | 0.156 |
| R-free | 0.18960 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.123 |
| High resolution limit [Å] | 1.999 |
| Number of reflections | 222659 |
| <I/σ(I)> | 14.91 |
| Completeness [%] | 93.2 |
| Redundancy | 12.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298.15 | 14% 1-6 Hexanediol, 10% PEG 3350, 100mM HEPEs, 10mM Adenosine Diphosphate, 5mM UDP-Xylose |
