5VR4

RNA octamer containing 2'-F-4'-OMe U.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 43 21 2
Unit cell lengths	44.387, 44.387, 85.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.481 - 1.500
R-factor	0.189
Rwork	0.186
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p4a
RMSD bond length	0.009
RMSD bond angle	1.804
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.067	0.795
Rpim	0.027	0.317
Number of reflections	26152
<I/σ(I)>	21.05	2.73
Completeness [%]	99.6	100
Redundancy	7.3	7.3
CC(1/2)		0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	0.5 mM RNA, 10 mM Cobalt(III) hexamine, 6 mM sodium chloride, 40 mM potassium chloride, 5% MPD, 20 mM sodium cacodylate equilibrated against reservoir containing 40% MPD