5VQP

Crystal structure of human pro-TGF-beta1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	P 6 2 2
Unit cell lengths	104.390, 104.390, 141.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.477 - 2.900
R-factor	0.2541
Rwork	0.252
R-free	0.29290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rjr
RMSD bond length	0.004
RMSD bond angle	0.641
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.170	6.000
Number of reflections	10672	753
<I/σ(I)>	12.32	0.48
Completeness [%]	99.7	100
Redundancy	10.5
CC(1/2)	0.999	0.150

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	289	100 mM MES pH6.3, 1.68 M Ammonium Sulfate, 11% Dioxane