5VNO

Crystal structure of Sec23a/Sec24a/Sec22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	158.046, 96.727, 126.796
Unit cell angles	90.00, 91.45, 90.00

Refinement procedure

Resolution	49.940 - 2.905
R-factor	0.2464
Rwork	0.244
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nut
RMSD bond length	0.005
RMSD bond angle	0.595
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	42151
<I/σ(I)>	15.9
Completeness [%]	98.6
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM HEPES PH 7.8, 0.5M NaCl and 12% PEG4K