5VNA
Crystal structure of human YEATS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-08-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.158, 79.926, 121.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.400 - 2.100 |
| R-factor | 0.17631 |
| Rwork | 0.174 |
| R-free | 0.22228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qrl |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.957 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 40747 | 4052 |
| <I/σ(I)> | 27.12 | 3 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 7.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.4 | 290 | 1.24 M ammonium sulfate, 100 mM CHES pH 9.4 |
