5VM9
Human Argonaute3 bound to guide RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.414, 68.414, 408.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.476 - 3.280 |
| R-factor | 0.1922 |
| Rwork | 0.187 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.846 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.480 | |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rpim | 0.086 | 0.408 |
| Number of reflections | 28649 | |
| <I/σ(I)> | 1.72 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM succinate-phosphateglycine buffer pH 4.4, 23% PEG2,000 and 4% PEG400 |
