5VL3
CD22 d1-d3 in complex with therapeutic Fab Epratuzumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-01 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.110, 90.220, 136.632 |
| Unit cell angles | 70.83, 80.81, 80.99 |
Refinement procedure
| Resolution | 39.345 - 3.100 |
| R-factor | 0.2806 |
| Rwork | 0.280 |
| R-free | 0.29530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.853 |
| Data scaling software | XPREP (2015/1) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.345 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.090 | 0.390 |
| Rpim | 0.090 | 0.390 |
| Number of reflections | 65863 | |
| <I/σ(I)> | 8.2 | 1.9 |
| Completeness [%] | 94.2 | 96.7 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.987 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20 mM Tris pH 8.0 and 150 mM NaCl, 5mg/mL |
