5VIG

Crystal structure of anti-Zika antibody Z006 bound to Zika virus envelope protein DIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	385.082, 385.082, 56.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.749 - 3.000
R-factor	0.2142
Rwork	0.212
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.844
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.270	96.270	3.040
High resolution limit [Å]	2.900	9.620	2.900
Rmerge	0.135	0.073	0.954
Rmeas	0.149	0.081	1.054
Rpim	0.062	0.034	0.441
Total number of observations	189075	5317	25287
Number of reflections	34940
<I/σ(I)>	7.5	16.7	1.7
Completeness [%]	99.0	97.5	99.6
Redundancy	5.4	5.4	5.4
CC(1/2)	0.990	0.984	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	10% isopropanol, 0.1M sodium citrate tribasic dihydrate pH 5.0, 26% PEG 400