5VGP

Fc fragment of human IgG1 antibody, from NIST mAb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	49.912, 79.959, 138.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.116
R-factor	0.20315
Rwork	0.201
R-free	0.24494
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3d0s
RMSD bond length	0.013
RMSD bond angle	1.656
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC (5.8.0155)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.170
High resolution limit [Å]	2.100	2.130
Number of reflections	30746
<I/σ(I)>	7.2
Completeness [%]	94.4	71
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	297	17% PEG 8K, 40 mM CaCl2 100 mM Na Hepes