5VFC
WDR5 bound to inhibitor MM-589
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-29 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.348, 58.559, 56.801 |
| Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
| Resolution | 15.550 - 1.640 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.550 |
| High resolution limit [Å] | 1.640 |
| Rmerge | 0.063 |
| Number of reflections | 35269 |
| <I/σ(I)> | 10 |
| Completeness [%] | 99.9 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Na Bis-Tris pH 6.5, 26% PEG 8000 and 0.1 M ammonium sulfate |
