5VCM

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase with bound UDP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-25
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	46.130, 139.330, 63.210
Unit cell angles	90.00, 111.08, 90.00

Refinement procedure

Resolution	43.044 - 1.599
R-factor	0.1874
Rwork	0.186
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.887
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.044	43.044	1.640
High resolution limit [Å]	1.600	7.150	1.600
Rmerge	0.106	0.057	1.408
Rmeas	0.114	0.061	1.514
Total number of observations	745960
Number of reflections	97795	1125	7094
<I/σ(I)>	10.97	29.08	1.41
Completeness [%]	99.7	98.8	98.3
Redundancy	7.628	7.354	7.347
CC(1/2)	0.998	0.997	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.7 M ammonium sulfate, 100 mM HEPES, pH 7.5, 5 mM UDP, 5 mM manganese chloride