5VBF

Crystal structure of succinate semialdehyde dehydrogenase from Burkholderia vietnamiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-22
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	106.620, 191.760, 219.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.166 - 2.350
R-factor	0.1476
Rwork	0.147
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jz4
RMSD bond length	0.007
RMSD bond angle	0.846
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX (dev_2689)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.166	48.166	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.158	0.063	0.517
Rmeas	0.172	0.069	0.564
Number of reflections	184886	2102	13707
<I/σ(I)>	10.69	23.76	3.93
Completeness [%]	98.7	90.6	99.7
Redundancy	6.379	6	6.335
CC(1/2)	0.991	0.995	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Rigaku Reagents Morpheus Screen G3: 0.020M Sodium Formate, 0.020M Ammonium Acetate, 0.020M Sodium Citrate, 0.020M Sodium Tartrate, 0.020M Sodium Oxamate, 0.1 M Imidazole/MES pH 6.5, 20% glycerol, 10% PEG 4000: BuviA.00020.v.B1.PS02593 at 14 mg/ml