5VBA
Structure of EspG1 chaperone from the type VII (ESX-1) secretion system determined with the assistance of N-terminal T4 lysozyme fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-05 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.140, 81.690, 160.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.540 - 2.270 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 4GBR & 4L4W |
RMSD bond length | 0.004 |
RMSD bond angle | 0.657 |
Data reduction software | XDS (November 3, 2014) |
Data scaling software | XSCALE (November 3, 2014 BUILT=20141118) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (DEV_2722) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.536 | 59.536 | 2.330 |
High resolution limit [Å] | 2.270 | 10.150 | 2.270 |
Rmerge | 0.097 | 0.048 | 1.110 |
Rmeas | 0.054 | 1.206 | |
Number of reflections | 39581 | 505 | 2874 |
<I/σ(I)> | 11.07 | 26.26 | 1.74 |
Completeness [%] | 99.8 | 97.1 | 100 |
Redundancy | 6.341 | 5.279 | 6.541 |
CC(1/2) | 0.996 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 0.1 M Bicine, pH 9.0, 1.0 M lithium chloride, 10% PEG6000 |