5VB2

Crystal structure of the NavAb voltage-gated sodium channel in a closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 2 21
Unit cell lengths	125.963, 129.853, 196.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.630 - 3.200
R-factor	0.235
Rwork	0.233
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.003
RMSD bond angle	0.701
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.627	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.101	0.811
Number of reflections	53226
<I/σ(I)>	12.8
Completeness [%]	98.9
Redundancy	5.3
CC(1/2)		0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.8 M ammonium sulfate and 100 mM sodium acetate, 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC):CHAPSO bicelles