5VA0
Glucocorticoid Receptor DNA Binding Domain in complex with AP-1 response element from VCAM-1 Promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.442, 96.280, 105.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.483 - 2.295 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hn5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 4.910 | 2.280 |
| Rmerge | 0.133 | 0.132 | 0.807 |
| Rmeas | 0.145 | 0.144 | 0.877 |
| Rpim | 0.056 | 0.056 | 0.338 |
| Total number of observations | 130117 | ||
| Number of reflections | 18797 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 96.7 | 99.6 | 90.1 |
| Redundancy | 6.9 | 6.6 | 6.7 |
| CC(1/2) | 0.983 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05 M Na Cacodylate (pH 7), 5 mM MgCl2, 1 mM Spermine, and 2 % t-Butanol |
