5V94
Pekin duck egg lysozyme isoform III (DEL-III), cubic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 95.950, 95.950, 95.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.340 - 1.650 |
| R-factor | 0.1885 |
| Rwork | 0.187 |
| R-free | 0.21430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iee |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.631 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.910 | 42.910 | 1.680 |
| High resolution limit [Å] | 1.650 | 8.900 | 1.650 |
| Rmerge | 0.100 | 0.050 | 1.015 |
| Rmeas | 0.103 | 0.052 | 1.043 |
| Rpim | 0.023 | 0.013 | 0.241 |
| Total number of observations | 4125 | 33403 | |
| Number of reflections | 35458 | ||
| <I/σ(I)> | 18.6 | 39.9 | 2.7 |
| Completeness [%] | 99.9 | 90.6 | 100 |
| Redundancy | 20.9 | 17.3 | 18.5 |
| CC(1/2) | 0.999 | 0.999 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.8 | 293 | 100 mM ammonium phosphate dibasic, 100 mM Tris, pH 8.8, 45% MPD |
