5V89

Structure of DCN4 PONY domain bound to CUL1 WHB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	118.930, 62.476, 38.580
Unit cell angles	90.00, 105.48, 90.00

Refinement procedure

Resolution	23.917 - 1.550
R-factor	0.1533
Rwork	0.151
R-free	0.19390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3TDU.pdb
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmerge	0.072	0.040	0.432
Number of reflections	36927
<I/σ(I)>	7.7
Completeness [%]	93.4	99.7	60.9
Redundancy	3.5	3.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	25% PEG3350, 0.2M LiSO4, 10mM TCEP, 10mM Bis-Tris Propane