5V7R

Cyrstal structure of anti-Tau antibody CBTAU-7.1 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97648
Spacegroup name	P 41
Unit cell lengths	83.903, 83.903, 109.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.171 - 2.300
R-factor	0.174
Rwork	0.171
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qeg 3fn0
RMSD bond length	0.009
RMSD bond angle	1.232
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.171	2.340
High resolution limit [Å]	2.300	2.300
Rpim	0.030	0.340
Number of reflections	33665
<I/σ(I)>	52.1	2.9
Completeness [%]	99.9	100
Redundancy	7.1	6.4
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.2 M ammonium dihydrogen phosphate, 20% PEG3350