5V68

Crystal structure of cell division protein FtsZ from Mycobacterium tuberculosis bounded via the T9 loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0781
Spacegroup name	P 21 21 21
Unit cell lengths	73.110, 180.850, 220.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.050 - 3.460
R-factor	0.24636
Rwork	0.243
R-free	0.30860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q1y
RMSD bond length	0.011
RMSD bond angle	1.470
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.050	3.550
High resolution limit [Å]	3.460	3.460
Rmerge	0.089	0.614
Rpim	0.053	0.366
Number of reflections	37287	2754
<I/σ(I)>	9.3	1.8
Completeness [%]	96.4	97.3
Redundancy	3.3	3.3
CC(1/2)	0.997	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M sodium citrate pH 5.6, 0.3M ammonium acetate, 15% PEG 4000. Protein concentration 3mg/ml. Incubated with 5mM SB-P17-A20