5V5Z
Structure of CYP51 from the pathogen Candida albicans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 91 |
Detector technology | CCD |
Collection date | 2015-02-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 91.940, 92.510, 101.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.505 - 2.900 |
R-factor | 0.2214 |
Rwork | 0.219 |
R-free | 0.26860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lxj |
RMSD bond length | 0.011 |
RMSD bond angle | 1.228 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.940 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.200 | 1.235 |
Number of reflections | 19796 | 3130 |
<I/σ(I)> | 8.6 | 1.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.5 | 7.6 |
CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | 0.5 M GLYCINE-NAOH, 35% PEG400, |