5V58
Crystal structure of human prolyl-tRNA synthetase in complex with Aze-SA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.766, 86.766, 108.773 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.540 - 2.590 |
| R-factor | 0.2291 |
| Rwork | 0.227 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hvc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.630 |
| High resolution limit [Å] | 2.590 | 7.020 | 2.590 |
| Rmerge | 0.057 | 0.053 | 0.337 |
| Number of reflections | 14793 | ||
| <I/σ(I)> | 19 | ||
| Completeness [%] | 96.9 | 98.4 | 86.5 |
| Redundancy | 10.2 | 9.4 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 9% PEG3350, 250 mM sodium nitrate, 100 mM 3-(1-pyridino)-1-propane sulfonate |
