5V4L
Cryptococcus neoformans adenylosuccinate lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2015-03-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.110, 93.500, 159.250 |
| Unit cell angles | 90.00, 97.54, 90.00 |
Refinement procedure
| Resolution | 39.437 - 2.100 |
| R-factor | 0.1594 |
| Rwork | 0.157 |
| R-free | 0.21140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ffx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.440 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 130683 |
| <I/σ(I)> | 2.78 |
| Completeness [%] | 98.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris/citric acid pH 6.5, 17% PEG 3350 and 0.12 M sodium chloride |
