5V3O

Cereblon in complex with DDB1 and CC-220

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-11
Detector	RAYONIX MX-225
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	111.029, 126.886, 172.487
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 3.200
R-factor	0.20693
Rwork	0.204
R-free	0.26733
RMSD bond length	0.009
RMSD bond angle	1.506
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.010
High resolution limit [Å]	3.200
Rmerge	0.089
Number of reflections	40911
<I/σ(I)>	19.8
Completeness [%]	100.0
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200mM NaCl, 20% PEG 3350