5V1M

Structure of human Usb1 with uridine 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	42.512, 53.219, 46.638
Unit cell angles	90.00, 107.04, 90.00

Refinement procedure

Resolution	44.590 - 1.470
R-factor	0.1611
Rwork	0.159
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4h7w
RMSD bond length	0.020
RMSD bond angle	1.860
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.590	1.500
High resolution limit [Å]	1.470	1.470
Number of reflections	65375	1621
<I/σ(I)>	12.8	1.2
Completeness [%]	99.3	99.8
Redundancy	6.7	6.7
CC(1/2)	0.999	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	20% w/v PEG 20,000, 20% v/v glycerol, 100 mM bis-tris base, 50 mM HCl, and 10 mM disodium 5' UMP