5V0H
RNA duplex with 2-MeImpG analogue bound-one binding site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2016-01-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 42.852, 42.852, 83.841 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.110 - 1.900 |
R-factor | 0.22942 |
Rwork | 0.227 |
R-free | 0.27244 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hbx |
RMSD bond length | 0.026 |
RMSD bond angle | 2.686 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.137 | 0.468 |
Number of reflections | 7273 | 702 |
<I/σ(I)> | 10.04 | 2.94 |
Completeness [%] | 97.4 | 95.4 |
Redundancy | 7.3 | 4.2 |
CC(1/2) | 0.957 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, and 0.16 M Ammonium tartrate dibasic, pH 7.0 |