5V0A
Crystal structure of human exonuclease 1 Exo1 (D225A) in complex with 5' recessed-end DNA (rVIII)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.739, 73.739, 179.714 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.370 - 2.380 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qea |
RMSD bond length | 0.009 |
RMSD bond angle | 0.986 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.074 | 0.635 |
Rmeas | 0.078 | 0.685 |
Number of reflections | 20768 | 998 |
<I/σ(I)> | 33.45 | 2.71 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 9.8 | 7 |
CC(1/2) | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |