5V08

Crystal structure of human exonuclease 1 Exo1 (D173A) in complex with 5' recessed-end DNA (rVI)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	74.259, 74.259, 179.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.455 - 2.812
R-factor	0.2186
Rwork	0.215
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.810	2.810
Rmerge	0.077	0.684
Rmeas	0.084	0.735
Number of reflections	12918	640
<I/σ(I)>	25.47	2.31
Completeness [%]	99.8	100
Redundancy	7.4	7.5
CC(1/2)	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000