5UZS

Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and P200

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97919
Spacegroup name	P 21 21 2
Unit cell lengths	111.124, 144.396, 87.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.087 - 2.367
R-factor	0.1734
Rwork	0.170
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Number of reflections	59812	2954
<I/σ(I)>	8	7.9
Completeness [%]	99.9	100
Redundancy	17.03	2.95
CC(1/2)		0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	289	0.05M acetate pH 5.2, 45% PEG 200