5UXB
Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium, apoenzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-02-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.933, 79.741, 99.132 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.753 - 2.794 |
R-factor | 0.2208 |
Rwork | 0.219 |
R-free | 0.25210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uxc |
RMSD bond length | 0.003 |
RMSD bond angle | 0.565 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2733) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rpim | 0.048 | 0.200 |
Number of reflections | 25067 | 771 |
<I/σ(I)> | 16.28 | 3.78 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.6 | 9.5 |
CC(1/2) | 0.962 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 2 M ammonium sulfate, 2% PEG 400, 0.1 M HEPES pH 7.5, 1% trehalose |