5UX0

X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA and target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	85.003, 130.993, 171.468
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.431 - 3.197
R-factor	0.2176
Rwork	0.215
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i4a
RMSD bond length	0.003
RMSD bond angle	0.655
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.431	47.430	3.370
High resolution limit [Å]	3.197	10.110	3.200
Rmerge	0.152	0.027	0.878
Rmeas	0.170	0.030	0.986
Rpim	0.076	0.014	0.443
Number of reflections	32393		4583
<I/σ(I)>	8.8
Completeness [%]	99.7	99.1	98.3
Redundancy	4.9	4.5	4.9
CC(1/2)	0.995	0.999	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	27% PEG 4,000, 0.1M MES, 275 mM KI