5UVW

BRD4_Bromodomain1-A1376855

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	141.438, 141.438, 132.614
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	122.490 - 2.140
R-factor	0.2056
Rwork	0.205
R-free	0.22570
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS (2.11.4)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall
Low resolution limit [Å]	122.500
High resolution limit [Å]	2.140
Rmerge	0.095
Number of reflections	43728
<I/σ(I)>	25.1
Completeness [%]	100.0
Redundancy	19.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0