5UTK
Crystal structure of the membrane proximal three fibronectin type III (FNIII) domains of Tie2 (Tie2[FNIIIa-c])
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.04, 0.97944, 0.97927, 0.94928 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.950, 52.026, 111.427 |
| Unit cell angles | 90.00, 117.49, 90.00 |
Refinement procedure
| Resolution | 49.328 - 2.500 |
| R-factor | 0.2266 |
| Rwork | 0.225 |
| R-free | 0.24720 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.901 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 5.720 | 2.500 |
| Rmerge | 0.106 | 0.065 | 0.562 |
| Total number of observations | 95446 | ||
| Number of reflections | 30201 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 97.4 | 97.4 | 85.1 |
| Redundancy | 3.2 | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 25-40% glycerol, 2.5-15% PEG 3K, 100 mM sodium acetate, 100 mM sodium phosphate, pH 4.5 |
